Biomedical – Biomedeine®

Biomedeine®  as cell protective agent in cell culture media

There are a lot of scientific studies having evidenced the cell protective effect of L-ergothioneine.

For example in the brain L-ergothioneine promote the differentiation of the neural stem cells NCS via mechanism different from its antioxidant action. Long term exposure of NCS to L-ergothioneine at 500μM for 9 days tended to increase expression of the brain-derived neurotrophic factor BDNF (1).

Another study shows the protective action of L-ergothioneine on human brain microvascular endothelial cells against glucose-induced oxidative stress (2).

Another study shows the protective action of L-ergothioneine on human lung epithelial cells from tobacco smoke-induced oxidative damage (3).

Biomedeine® consists in 99.5% pure L-ergothioneine.

Biomedeine® as pure L-ergothioneine can be used as cell protective agent in cell culture media

1. Quality

Biomedeine® has a very high water solubility and good heat stability, regardless of formulation

Biomedeine®  is an odorless white powder

Biomedeine® is a highly pure source of L-ergothioneine (> 99.5%)

Manufactured via a proprietary process using a biomimetic and sustainable technology (WO 2011042480-A1). Reagents are all non GMO; none are of either human or animal origin

Food cGMP quality product

2. Sourcing

Biomedeine® is packaged :

a/ in powder:

– 1-gm plastic bottle

– 10-gm plastic bottle

– 250-gm plastic bottle

b/ in water solution:

– 4.36mM aqueous solution: ready to use is also available on request

Biomedeine® as cell protective agent in liquid media for organ transplantation

  • Hepatic Ischemia-reperfusion (IR) is a common problem encountered in a variety of clinical conditions such as liver transplantation, hepatic failure after shock, and liver surgery. IR causes functional and structural damage to liver cells.

Recent data show that reoxygenation causes a remarkable production of HSP70. Overproduction of HSP in the liver induced by whole-body hyperthermia was shown to increase resistance to IR injury. One proposed mechanism of IR injury is ROS-mediated lipid peroxidation of cellular membranes. Among the systemic consequences of reperfusion, lipid peroxidation is probably the most damaging effect of ROS; it results in structural and functional derangement and eventually cell death.

Recently it has been suggested that the most likely explanation for antioxidant activity of L-ergothioneine is that L-ergothioneine acted synergistically with ischemic stress in the liver on production of HSP70. Further laboratory studies examining the potential mechanisms of L-ergothioneine need to be carried out in liver IR injury.

L-ergothioneine pretreatment protects the liver from IR injury by over-expression of HSP and the subsequent suppressing the lipid peroxidation (4). Pretreatment with L-ergothioneine for 3 weeks induced HSP70 expression in the rat liver. EGT pretreatment also improves IR-induced liver injury and mortality.

 

  • Kidney function can be affected by numerous factors including oxidative stress.

The Fenton chemistry Fe-NTA model is a unique model for studying the ability of dietary factors to afford antioxidant protection to the kidney. Degeneration of renal proximal tubular cells can occur at 1–3 h after single administration of Fe-NTA with necrosis prominent at 24 h.47 Lipid peroxidation is induced 30 min after administration, and continues for about 6 h (peaking at 1–2 h) whilst aldehyde-modified proteins are increased up to 48 h after single administration. Under the experimental model, maximum oxidative damage is reached at 3 h following the acute injection of Fe-NTA. At 4 h there is increased synthesis of the fatty acids in response to the oxidative insult. Thus orally administered L-Ergo is actively absorbed and affords protection to the kidney.

L-Ergo provides protection against the Fe-NTAdependent decrease in the kidney and liver endogenous levels of docosahexaenoic acid (with the liver protected to a lesser extent). L-Ergo protected the levels of eicosapentaenoic acid in both kidney and liver (5).

In 2017, Seebeck et al. evidence that some anaerobic bacteria own the genes for ergothioneine production (6). It seems that ergothioneine had an important role under anaerobic conditions. Then a putative hypothesis could be that ergothioneine could contribuate to the cell protection during the ischemic phase of the IR event and allow to a non-destructive reoxygenation of the tissue.

Biomedeine® as pure L-ergothioneine can be used as cell protective agent in preservation media for organ transplantation

1. Quality

Biomedeine® has a very high water solubility and good heat stability, regardless of formulation

Biomedeine®  is an odorless white powder

Biomedeine® is a highly pure source of L-ergothioneine (> 99.5%)

Manufactured via a proprietary process using a biomimetic and sustainable technology (WO 2011042480-A1). Reagents are all non GMO; none are of either human or animal origin

Food cGMP quality product

2. Sourcing

Biomedeine® is packaged :

a/ in powder:

– 1-gm plastic bottle

– 10-gm plastic bottle

– 250-gm plastic bottle

b/ in water solution:

– 4.36mM aqueous solution: ready to use is also available on request

Biomedeine® as cell protective agent in sperm conservation

Oxidative stress is thus better defined as the fail in the regulation of redox signaling due either to overproduction of reactive oxygen species (ROS) and the result of mitochondrial malfunction, or exhaustion of regulatory mechanisms. Several potential sources can be responsible for ROS production in the  spermatozoa, including the spermatozoa itself and contaminating cells in the  ejaculate. Dead spermatozoa are a major source of ROS, frequently overlooked in reproductive technologies.

Nowadays it is considered that the main source of ROS is the electron leakage in the mitochondrial electron transport chain (ETC). It is the result of “a dysfunction of electron transfer reactions leading to oxidant/antioxidant imbalance and oxidative damage to macromolecules ».

Numerous evidence points to mitochondria as the hallmark of fertile spermatozoa and then defective mitochondria may represent a hallmark of male infertility. Evidences of mitophagy in human sperm were described, suggesting that activation of mitophagy is a mechanism that maintains proper sperm function.

The spermatozoa itself also has antioxidant defenses, including glutathione, and  other enzymatic antioxidant defenses. Redox regulation is tightly regulated in the spermatozoa, with interactions between spermatic metabolism, mitochondrial production and scavenging of ROS. A summary of current knowledge on redox regulation in spermatozoa is presented in figure 1. Many factors can deregulate this complex network in humans, including aging, exposure to toxins, particularly alcohol and tobacco, poor diet, lack of physical activity and systemic diseases including obesity and diabetes. Also, current sperm biotechnologies such as cryopreservation cause redox deregulation of spermatozoa, mainly through a severe mitochondrial osmotic stress. Deregulation of redox homeostasis has a profound impact on sperm physiology and fertility, all spermatic compartments and function may be affected, moreover impacts on the embryo and the offspring may also occur

It is well recognized that equine seminal plasma is rich in antioxidants. The function and chemical composition of the seminal plasma is very complicated and varies according to accessory gland contributions and fertility status of the male. Seminal plasma secretion is under endocrine control by FSH, LH and testosterone in the testes and the anterior pituitary gland (7). Biological components such as choline, citric acid, fructose, inositol and ergothioneine, are present in abnormal amounts in seminal plasma when compared to other body fluids and are specific for the regulation of sperm function (8).

L-ergothioneine supplement significantly reduced oxidative stress by decreasing MDA levels and increasing SOD, GPx and CAT activities. L-ergothioneine supplement shows a normal histological arrangement similar to the control when administered alone. It was able to prevent these damages via its antioxidant, cytoprotectant and steroidogenic ability thereby hindering the processes of peroxidation of the polyunsaturated fatty acids in the membrane of testicular tissues, protecting the seminiferous tubule and leydig cell, thereby maintaining the integrity of the testis.

L-ergothioneine supplement has protective effects on cisplatin-induced testicular dysfunction via its antioxidant and cyto-protectant activities. Thus, it has the potential for use as therapeutic adjuvants against reproductive toxicity in patients undergoing Cisplatin therapy.

Biomedeine® as pure L-ergothioneine can be used as cell protective agent in sperm conservation

1; Quality

Biomedeine® has a very high water solubility and good heat stability, regardless of formulation

Biomedeine®  is an odorless white powder

Biomedeine® is a highly pure source of L-ergothioneine (> 99.5%)

Manufactured via a proprietary process using a biomimetic and sustainable technology (WO 2011042480-A1). Reagents are all non GMO; none are of either human or animal origin

Food cGMP quality product

2. Sourcing

Biomedeine® is packaged :

a/ in powder:

– 1-gm plastic bottle

– 10-gm plastic bottle

– 250-gm plastic bottle

b/ in water solution:

– 4.36mM aqueous solution: ready to use is also available on request

 

  1. Ishimoto, 2016
  2. Li, 2014
  3. Nickel, 2016
  4. Bedirli, 2004
  5. Deiana, 2004
  6. Seebeck, 2017
  7. Lambrechts, 1996
  8. Barrios et al., 2000